The common Repository of Adventitious Proteins, cRAP (pronounced "cee-RAP"), is
an attempt to create a list of proteins commonly found in proteomics experiments that are present
either by accident or through unavoidable contamination of protein samples.
The types of proteins included fall into three general classes:
- common laboratory proteins;
- proteins added by accident through dust or physical contact; and
- proteins used as molecular weight or mass spectrometry quantitation standards.
Version 1.0 of cRAP was based on NCBI protein sequence entries, drawn from "nr". The current version
(2012.01.01) was based on UNIPROT protein sequence entries. This change was made to take advantage of the higher information
content in the curated entries in the UNIPROT-knowledgebase.
This list is based on an analysis of the current version of GPMDB, as well
as suggestions by users. If you would like to have other proteins included in this list, please contact us by
email with your suggestions.
The current version of cRAP in FASTA format can be obtained from the GPM FTP site, using the URL
ftp://ftp.thegpm.org/fasta/cRAP.
1. Laboratory proteins:
|
Id |
Description |
Reason |
1. |
ALBU_BOVIN |
(P02769) Serum albumin precursor (Allergen Bos d 6) (BSA) |
Reagent and lab contaminant |
2. |
CTRA_BOVIN |
(P00766) Chymotrypsinogen A (EC 3.4.21.1) [Contains: Chymotrypsin A chain A; Chymotrypsin A chain B; Chymotrypsin A chain C] |
Reagent |
3. |
CTRB_BOVIN |
(P00767) Chymotrypsinogen B (EC 3.4.21.1) [Contains: Chymotrypsin B chain A; Chymotrypsin B chain B; Chymotrypsin B chain C] |
Reagent |
4. |
LALBA_BOVIN |
(P00711) Alpha-lactalbumin precursor (Lactose synthase B protein) (Allergen Bos d 4) |
Reagent and lab contaminant |
5. |
LYSC_LYSEN |
(Q7M135) Lysyl endopeptidase (EC 3.4.21.50) (Lys-C) |
Reagent |
6. |
PEPA_BOVIN |
(P00792) Pepsin A precursor (EC 3.4.23.1) |
Reagent |
7. |
PEPA_PIG |
(P00791) Pepsin A precursor (EC 3.4.23.1) |
Reagent |
8. |
PEPB_PIG |
(Q10735) Pepsin B precursor (EC 3.4.23.2) (Parapepsin I) (Fragment) |
Reagent |
9. |
PEPC_PIG |
(P30879) Gastricsin precursor (EC 3.4.23.3) (Pepsinogen C) (Fragment) |
Reagent |
10. |
SSPA_STAAU |
(P04188) Glutamyl endopeptidase precursor (EC 3.4.21.19) (Staphylococcal serine proteinase) (V8 protease) (V8 proteinase) (Endoproteinase Glu-C) |
Reagent |
11. |
TRY1_BOVIN |
(P00760) Cationic trypsin precursor (EC 3.4.21.4) (Beta-trypsin) [Contains: Alpha-trypsin chain 1; Alpha-trypsin chain 2] (Fragment) |
Reagent |
12. |
TRY2_BOVIN |
(Q29463) Anionic trypsin precursor (EC 3.4.21.4) |
Reagent |
13. |
TRYP_PIG |
(P00761) Trypsin precursor (EC 3.4.21.4) |
Reagent |
14. |
CAS1_BOVIN |
(P02662) Alpha-S1-casein precursor |
Reagent and lab contaminant |
15. |
CAS2_BOVIN |
(P02663) Alpha-S2-casein precursor [Contains: Casocidin-1 (Casocidin-I)] |
Reagent and lab contaminant |
16. |
CASB_BOVIN |
(P02666) Beta-casein precursor |
Reagent and lab contaminant |
17. |
CASK_BOVIN |
(P02668) Kappa-casein precursor [Contains: Casoxin C; Casoxin 6; Casoxin A; Casoxin B; Casoplatelin] |
Reagent and lab contaminant |
18. |
GFP_AEQVI |
(P42212) Aequorea victoria Green fluorescent protein |
Reagent |
19. |
PLMP_GRIFR |
(P81054) Peptidyl-Lys metalloendopeptidase precursor - Grifola frondosa (LysN) |
Reagent |
2. Dust/contact proteins:
|
Id |
Description |
Reason |
1. |
AMYS_HUMAN |
(P04745) Salivary alpha-amylase precursor (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase) |
Human saliva |
2. |
K1C10_HUMAN |
(P13645) Keratin, type I cytoskeletal 10 (Cytokeratin-10) (CK-10) (Keratin-10) (K10) |
Human skin |
3. |
K1C9_HUMAN |
(P35527) Keratin, type I cytoskeletal 9 (Cytokeratin-9) (CK-9) (Keratin-9) (K9) |
Human skin |
4. |
K2C1_HUMAN |
(P04264) Keratin, type II cytoskeletal 1 (Cytokeratin-1) (CK-1) (Keratin-1) (K1) (67 kDa cytokeratin) (Hair alpha protein) |
Human skin |
5. |
K22E_HUMAN |
(P35908) Keratin, type II cytoskeletal 2 epidermal (Cytokeratin-2e) (K2e) (CK 2e) |
Human skin |
6. |
K1H1_HUMAN |
(Q15323) Keratin, type I cuticular Ha1 (Hair keratin, type I Ha1) |
Human hair |
7. |
K1H2_HUMAN |
(Q14532) Keratin, type I cuticular Ha2 (Hair keratin, type I Ha2) |
Human hair |
8. |
K1H4_HUMAN |
(O76011) Keratin, type I cuticular Ha4 (Hair keratin, type I Ha4) |
Human hair |
9. |
K1H5_HUMAN |
(Q92764) Keratin, type I cuticular Ha5 (Hair keratin, type I Ha5) |
Human hair |
10. |
K1H6_HUMAN |
(O76013) Keratin, type I cuticular Ha6 (Hair keratin, type I Ha6) |
Human hair |
11. |
K1H7_HUMAN |
(O76014) Keratin, type I cuticular Ha7 (Hair keratin, type I Ha7) |
Human hair |
12. |
K1H8_HUMAN |
(O76015) Keratin, type I cuticular Ha8 (Hair keratin, type I Ha8) |
Human hair |
13. |
K1HA_HUMAN |
(O76009) Keratin, type I cuticular Ha3-I (Hair keratin, type I Ha3-I) |
Human hair |
14. |
K1HB_HUMAN |
(Q14525) Keratin, type I cuticular Ha3-II (Hair keratin, type I Ha3-II) |
Human hair |
15. |
KRHB1_HUMAN |
(Q14533) Keratin, type II cuticular Hb1 (Hair keratin, type II Hb1) (ghHKb1) (ghHb1) (MLN 137) |
Human hair |
16. |
KRHB2_HUMAN |
(Q9NSB4) Keratin, type II cuticular Hb2 (Hair keratin, type II Hb2) |
Human hair |
17. |
KRHB3_HUMAN |
(P78385) Keratin, type II cuticular Hb3 (Hair keratin, type II Hb3) |
Human hair |
18. |
KRHB4_HUMAN |
(Q9NSB2) Keratin, type II cuticular Hb4 (Hair keratin, type II Hb4) |
Human hair |
19. |
KRHB5_HUMAN |
(P78386) Keratin, type II cuticular Hb5 (Hair keratin, type II Hb5) |
Human hair |
20. |
KRHB6_HUMAN |
(O43790) Keratin, type II cuticular Hb6 (Hair keratin, type II Hb6) (ghHb6) |
Human hair |
21. |
K1C15_SHEEP |
(O77727) Keratin, type I cytoskeletal 15 (Cytokeratin-15) (CK-15) (Keratin-15) (K15) |
Sheep wool |
22. |
K1M1_SHEEP |
(P02534) Keratin, type I microfibrillar 48 kDa, component 8C-1 (Low-sulfur keratin) |
Sheep wool |
23. |
K1M2_SHEEP |
(P25690) Keratin, type I microfibrillar, 47.6 kDa (Low-sulfur keratin) |
Sheep wool |
24. |
K2M1_SHEEP |
(P02539) Keratin, type II microfibrillar (Low-sulfur keratin) (Fragment) |
Sheep wool |
25. |
K2M2_SHEEP |
(P15241) Keratin, type II microfibrillar, component 7C |
Sheep wool |
26. |
K2M3_SHEEP |
(P25691) Keratin, type II microfibrillar, component 5 |
Sheep wool |
27. |
KRA33_SHEEP |
(P02444) Keratin, high sulfur matrix protein, IIIB3 |
Sheep wool |
28. |
KRA34_SHEEP |
(P02445) Keratin, high sulfur matrix protein, IIIB4 |
Sheep wool |
29. |
KRA3A_SHEEP |
(P02443) Keratin, high-sulfur matrix protein, IIIA3A |
Sheep wool |
30. |
KRA3_SHEEP |
(P02441) Keratin, high-sulfur matrix protein, IIIA3 |
Sheep wool |
31. |
KRA61_SHEEP |
(Q02958) Keratin, glycine/tyrosine-rich of hair |
Sheep wool |
32. |
KRB2A_SHEEP |
(P02438) Keratin, high-sulfur matrix protein, B2A |
Sheep wool |
33. |
KRB2B_SHEEP |
(P02439) Keratin, high-sulfur matrix protein, B2B |
Sheep wool |
34. |
KRB2C_SHEEP |
(P02440) Keratin, high-sulfur matrix protein, B2C |
Sheep wool |
35. |
KRB2D_SHEEP |
(P08131) Keratin, high-sulfur matrix protein, B2D |
Sheep wool |
36. |
KRUC_SHEEP |
(P26372) Keratin, ultra high-sulfur matrix protein (UHS keratin) |
Sheep wool |
37. |
SRPP_HEVBR |
(O82803) Small rubber particle protein - Hevea brasiliensis |
Latex gloves |
38. |
REF_HEVBR |
(P15252) Rubber elongation factor protein - Hevea brasiliensis |
Latex gloves |
3. Molecular weight markers/Standard proteins:
|
Id |
Description |
Reason |
1. |
CYC_HORSE |
(P00004) Horse heart cytochrome C |
Molecular weight marker/mass spec. standard |
2. |
CAH2_BOVIN |
(P00921) Bovine carbonic anhydrase 2 (EC 4.2.1.1) |
Molecular weight marker |
3. |
ADH1_YEAST |
(P00330) Yeast alcohol dehydrogenase 1 |
Molecular weight marker |
4. |
ALDOA_RABIT |
(P00883) Rabbit fructose-bisphosphate aldolase A |
Molecular weight marker |
5. |
LYSC_CHICK |
(P00698) Hen egg lysozyme C precursor |
Molecular weight marker/mass spec. standard |
6. |
MYG_HORSE |
(P68082) Horse heart myoglobin |
Molecular weight marker/mass spec. standard |
7. |
OVAL_CHICK |
(P01012) Hen egg ovalbumin |
Molecular weight marker |
8. |
BGAL_ECOLI |
(P00722) E. coli beta-galactosidase (E.C. 3.2.1.23) |
Molecular weight marker |
9. |
DHE3_BOVIN |
(P00366) Bovine glutamate dehydrogenase 1, mitochondrial precursor (E.C. 1.4.1.3) |
Molecular weight marker |
4. Sigma-Aldrich Universal Protein Standard:
|
Id |
Description |
Reason |
1. |
ALBU_HUMAN |
(P02768) Serum albumin 66,393 Recombinant Pichia pastoris |
UPS |
2. |
ANT3_HUMAN |
(P01008) Antithrombin-III 49,033 Plasma Glycosylation |
UPS |
3. |
ANXA5_HUMAN |
(P08758) Annexin A5 35,782 Placenta Acetylation |
UPS |
4. |
B2MG_HUMAN |
(P61769) Beta-2-microglobulin 11,729 Urine |
UPS |
5. |
BID_HUMAN |
(P55957) BH3 interacting domain death agonist [BID] 21,978 Recombinant E. coli |
UPS |
6. |
CAH1_HUMAN |
(P00915) Carbonic anhydrase 1 28,738 Erythrocytes Acetylation |
UPS |
7. |
CAH2_HUMAN |
(P00918) Carbonic anhydrase 2 29,095 Erythrocytes Acetylation |
UPS |
8. |
CATA_HUMAN |
(P04040) Catalase 59,583 Erythrocytes |
UPS |
9. |
CATD_HUMAN |
(P07339) Cathepsin D 26,624 Liver Glycosylation |
UPS |
10. |
CATG_HUMAN |
(P08311) Cathepsin G 26,751 Sputum Glycosylation |
UPS |
11. |
CO5_HUMAN |
(P01031) Complement C5 [Complement C5a] 8,266 Recombinant E. coli |
UPS |
12. |
CRP_HUMAN |
(P02741) C-reactive protein 23,030 Plasma |
UPS |
13. |
CYB5_HUMAN |
(P00167) Cytochrome b5 16,021 Recombinant E. coli 6-His |
UPS |
14. |
CYC_HUMAN |
(P99999) Cytochrome c [Apocytochrome c] 11,608 Recombinant E. coli |
UPS |
15. |
EGF_HUMAN |
(P01133) Epidermal growth factor 6,211 Recombinant E. coli |
UPS |
16. |
FABPH_HUMAN |
(P05413) Fatty acid-binding protein 14,716 Plasma Acetylation,Phosphorylation |
UPS |
17. |
GELS_HUMAN |
(P06396) Gelsolin 82,954 Plasma Phosphorylation |
UPS |
18. |
GSTA1_HUMAN |
(P08263) Glutathione S-transferase A1 [GST A1-1] 25,482 Recombinant E. coli |
UPS |
19. |
GSTP1_HUMAN |
(P09211) Glutathione S-transferase P [GST] 23,220 Placenta |
UPS |
20. |
HBA_HUMAN |
(P69905) Hemoglobin alpha chain 15,127 Erythrocytes |
UPS |
21. |
HBB_HUMAN |
(P68871) Hemoglobin beta chain 15,867 Erythrocytes,Acetylation,Nitrosylation,Glycosylation |
UPS |
22. |
IGF2_HUMAN |
(P01344) Insulin-like growth factor II 7,464 Recombinant E. coli |
UPS |
23. |
IL8_HUMAN |
(P10145) Interleukin-8 8,381 Recombinant E. coli |
UPS |
24. |
KCRM_HUMAN |
(P06732) Creatine kinase M-type [CK-MM] 43,070 Heart |
UPS |
25. |
LALBA_HUMAN |
(P00709) Alpha-lactalbumin 14,070 Milk Glycosylation |
UPS |
26. |
LEP_HUMAN |
(P41159) Leptin 16,024 Recombinant E. coli |
UPS |
27. |
LYSC_HUMAN |
(P61626) Lysozyme C 14,692 Milk |
UPS |
28. |
MYG_HUMAN |
(P02144) Myoglobin 17,051 Heart |
UPS |
29. |
NEDD8_HUMAN |
(Q15843) Neddylin [Nedd8] 9,071 Recombinant E. coli |
UPS |
30. |
NQO1_HUMAN |
(P15559) NAD(P)H dehydrogenase [quinone] 1[DT Diaphorase] 30,984 Recombinant E. coli |
UPS |
31. |
NQO2_HUMAN |
(P16083) Ribosyldihydronicotinamide dehydrogenase(quinone) [Quinone oxidoreductase 2 or NQO2] 25,817 Recombinant E. coli |
UPS |
32. |
PDGFB_HUMAN |
(P01127) Platelet-derived growth factor B chain 12,286 Recombinant E. coli |
UPS |
33. |
PPIA_HUMAN |
(P62937) Peptidyl-prolyl cis-trans isomerase A [Cyclophilin A] 17,947 Recombinant E. coli |
UPS |
34. |
PRDX1_HUMAN |
(Q06830) Peroxiredoxin 1 22,106 Recombinant E. coli |
UPS |
35. |
RASH_HUMAN |
(P01112) GTPase HRas [Ras protein] 21,292 Recombinant E. coli |
UPS |
36. |
RETBP_HUMAN |
(P02753) Retinol-binding protein 21,065 Urine |
UPS |
37. |
SODC_HUMAN |
(P00441) Superoxide dismutase [Cu-Zn] 15,800 Erythrocytes Acetylation |
UPS |
38. |
SUMO1_HUMAN |
(P63165) Small ubiquitin-related modifier 1 [SUMO-1] 37,420 Recombinant E. coli GST |
UPS |
39. |
SYH_HUMAN |
(P12081) Histidyl-tRNA synthetase [Jo-1] 58,223 Recombinant E. coli |
UPS |
40. |
TAU_HUMAN |
(P10636) Microtubule-associated protein tau [Tau protein] 46,810 Recombinant E. coli 6-His |
UPS |
41. |
THIO_HUMAN |
(P10599) Thioredoxin 12,424 Recombinant E. coli 6-His |
UPS |
42. |
TNFA_HUMAN |
(P01375) Tumor necrosis factor [TNF-alpha] 17,350 Recombinant E. coli |
UPS |
43. |
TRFE_HUMAN |
(P02787) Serotransferrin [Apotransferrin] 75,143 Plasma Glycosylation |
UPS |
44. |
TRFL_HUMAN |
(P02788) Lactotransferrin 78,289 Milk Glycosylation |
UPS |
45. |
UB2E1_HUMAN |
(P51965) Ubiquitin-conjugating enzyme E2 E1 [UbcH6] |
UPS |
46. |
UBE2C_HUMAN |
(O00762) Ubiquitin-conjugating enzyme E2 C [UbcH10] 20,473 Recombinant E. coli 6-His |
UPS |
47. |
UBE2I_HUMAN |
(P63279) Ubiquitin-conjugating enzyme E2 I [UbcH9] 22,907 Recombinant E. coli |
UPS |
48. |
RS27A_HUMAN |
(P62979) Ubiquitin-40S ribosomal protein S27a 9,387 Recombinant E. coli 6-His |
UPS |
5. Common viral contaminants:
|
Id |
Description |
Reason |
1. |
GAG_SCVLA |
(P32503) Major coat protein, Saccharomyces cerevisiae virus L-A (ScV-L-A) (ScVL1) |
virus infects yeast cultures |
|